Tree of Life depicts the evolutionary journey from the Last Universal Common Ancestor (LUCA) to the three domains of life—Bacteria, Archaea, and Eukaryota. LUCA is represented at the base of the tree as a kaleidoscope of colours, symbolizing the origins of life, with branching hues of green for Bacteria, yellow for Archaea, and purple for Eukaryota. 

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The artwork spans multiple canvases, separated intentionally to represent the gaps in scientific knowledge and the everchanging nature of our understanding. The gradient background shifts from deep blue, reflecting the ancient origins of life, to lighter shades symbolizing the present and an unknown future. Flecks of scattered colour evoke species lost to evolution or yet to be discovered. 

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Lafirenze meticulously developed her technique through trial and error, troubleshooting variables such as paint consistency and flow to achieve the desired effects. The physical scale of the piece posed challenges, requiring significant time and physical effort, but also highlighting her dedication to translating scientific complexity into visual form​​.